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May be used in the biotinylation of proteins and peptides. Typically coupled to primary amine in the pH range 6.5-8.5.

Biotinylation reagent incorporating an extended 14-atom spacer can$nreduce steric hindrance in binding avidin or streptavidin to some$nbiotinylated compounds and increase sensitivity for detection. Typically$ncoupled to primary amine in the pH range 6.5-8.5.